The use of chitin binding proteins for glycoprotein analysis

The focus of the pharmaceutical industry has dramatically shifted in the past number of years. Traditional drugs were synthesised using chemical reactions have been replaced by recombinant glycoprotein molecules. These potential recombinant glycoprotein therapeutics display oligosaccharide structures on their surfaces that are recognised by their target host. The specific glycan moieties on the surface of the molecules vary dramatically and have a large impact on the efficacy of the drug. The development of bioanalytical tools to identify and separate the species of glyco-forms present in a preparation of the glycoprotein therapeutic will significantly help to advance the quality and effectiveness of recombinant glycoprotein molecules. Traditionally lectins, isolated from plants, had been used to profile sugar species displayed on glycoproteins. I have explored the use of prokaryotic chitin binding proteins (CBPs) to investigate structures on glycoproteins

Eras of electric vehicles: electric mobility on the Verge. Focus Attention Scale

Daily or casual passenger vehicles in cities have negative burden on our finite world. Transport sector has been one of the main contributors to air pollution and energy depletion. Providing alternative means of transport is a promising strategy perceived by motor manufacturers and researchers. The paper presents the battery electric vehicles-BEVs bibliography that starts with the early eras of invention up till 2015 outlook. It gives a broad overview of BEV market and its technology in a chronological classification while sheds light on the stakeholders’ focus attentions in each stage, the so called, Focus-Attention-Scale-FAS. The attention given in each era is projected and parsed in a scale graph, which varies between micro, meso, and macro-scale. BEV-system is on the verge of experiencing massive growth; however, the system entails a variety of substantial challenges. Observations show the main issues of BEVsystem that require more attention followed by the authors’ recommendations towards an emerging market

The Owl

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The University of Saskatchewan Native Law Centre

In the minds of many, the Native Law Centre is identified with the Program of Legal Studies for Native People, a prerequisite orientation program for native students admitted to law school in the discretionary category. The association of the two is inevitable since the Native Law Centre has its origins in the Program of Legal Studies for Native People and continues to offer the course each summer. However, the Centre provides other resources and conducts several activities other than the summer program (as the Program of Legal Studies for Native People is often called). This note will give a brief account of the Native Law Centre\u27s growth out of the summer program, outline the Centre\u27s activities including the summer program, and look at some of the activities planned for the Centre in the coming years

Suddon Oak Death Syndrome

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Following the relaxation dynamics of photoexcited aniline in the 273-266 nm region using time-resolved photoelectron imaging

Scanning tunnelling microscopy (STM) and Auger electron spectroscopy (AES) have been used to investigate the growth morphology of ultra-thin Pb films on the Ni3Al(111) face at room temperature. A previous study [K. Miśków and A. Krupski Appl Surf Sci 273, 2013, 554] using low-energy electron diffraction (LEED) and real time Auger intensity recording has demonstrated that an initial two-dimensional growth of the first Pb monolayer thick ‘wetting layer’ takes place. With further deposition and for T = 300 K, flat three atomic-layer-high islands are grown. Above 350 K, the Stranski–Krastanov growth mode was observed. In the current study, the analysis of STM measurements indicate and confirm that for coverage θ = 1.0 ML two-dimensional growth of the first Pb monolayer took place. Above θ > 1.0 ML, a three-dimensional growth of the Pb islands was observed with a strongly preferred atomic-scale ‘magic height (N),’ hexagonal shape and flat-tops. At coverage θ = 3.5 ML, only islands containing N = 3, 5, 7 and 11 atomic layers of Pb are observed. At the higher coverage θ = 5.5 ML, three types of regular hexagonal islands with side lengths of 25, 30 and 45 nm are observed. Furthermore, three different island adsorption configurations rotated by 10° ± 1° and 30° ± 6° with respect to each other were observed. After an annealing at T = 400 K of 5.5 ML of lead deposited at RT on the Ni3Al(111) the morphology of the surface changes. Post-anneal, islands of Pb are observed above the ‘wetting layer’ with an estimated average size and diameter of 768 ± 291 nm2 and 38.17 ± 6.56 nm and constant uniform height of two atomic layers (N = 2)

Fully Coherent X-ray Pulses from a Regenerative Amplifier Free Electron Laser

We propose and analyze a novel regenerative amplifier free electron laser (FEL) to produce fully coherent x-ray pulses. The method makes use of narrow-bandwidth Bragg crystals to form an x-ray feedback loop around a relatively short undulator. Self-amplified spontaneous emission (SASE) from the leading electron bunch in a bunch train is spectrally filtered by the Bragg reflectors and is brought back to the beginning of the undulator to interact repeatedly with subsequent bunches in the bunch train. The FEL interaction with these short bunches not only amplifies the radiation intensity but also broadens its spectrum, allowing for effective transmission of the x-rays outside the crystal bandwidth. The spectral brightness of these x-ray pulses is about two to three orders of magnitude higher than that from a single-pass SASE FEL.Comment: 11 pages, 6 figure